the viability of mouse spermatogonial germ cells on a novel scaffold, containing human serum albumin and calcium phosphate nanoparticles

Authors

mona yadegar

seyed hossein hekmatimoghaddam

saeide nezami saridar

ali jebali

abstract

background: in spermatogenesis, spermatogonial cells differentiate to the haploid gametes. it has been shown that spermatogenesis can be done at in vitro condition. in vitro spermatogenesis may provide an open window to treat male infertility. objective: the aim of this study was to evaluate the effects of a novel scaffold containing human serum albumin (hsa)/tri calcium phosphate nanoparticles (tcp nps) on the mouse spermatogonial cell line (scl). materials and methods: first, tcp nps were synthesized by reaction of calcium nitrate and diammonium phosphate at ph 13. then, serial concentrations of tcp nps were separately added to 500 mg/ml hsa, and incubated in the 100oc water for 30 min. in the next step, each scaffold was cut (2×2mm), placed into sterile well of microplate, and then incubated for 1, 2, and 3 days at 37oc with mouse scl. after incubation, the cytotoxicity of the scaffolds was evaluated by different tests including 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (mtt) assay, lactate dehydrogenase (ldh) assay, vital staining, and cell counting. on the other hand, the release of tcp nps and hsa from the scaffolds was measured. results: based on microscopic observation, the size of cavities for all scaffolds was near 200-500 μm, and the size of tcp nps was near 50-100 nm. all toxicity tests showed that the increase of tcp concentration in the scaffold did not affect mouse scl. it means that the percentage of cell viability, ldh release, vital cells, and cell quantity was 85%, 105%, 90%, and 110%, respectively. but, the increase of incubation time led to increase of ldh release (up to 115%) and cell count (up to 115%). also, little decrease of cell viability and vital cells was seen when incubation time was increased. here, no release of tcp nps and hsa was seen after increase of tcp concentration and incubation time. conclusion: it can be concluded that the increase of tcp concentration in hsa/ tcp nps scaffold does not lead to cytotoxicity. on the other hand, the increase of incubation time leads to increase of mouse scl cell death. in this study, it was found that tcp nps and hsa could not release from the scaffolds. in future, both proliferation and differentiation of mouse scl on hsa/tcp nps scaffold must be checked over more wide incubation times.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

The viability of mouse spermatogonial germ cells on a novel scaffold, containing human serum albumin and calcium phosphate nanoparticles

BACKGROUND In spermatogenesis, spermatogonial cells differentiate to the haploid gametes. It has been shown that spermatogenesis can be done at in vitro condition. In vitro spermatogenesis may provide an open window to treat male infertility. OBJECTIVE The aim of this study was to evaluate the effects of a novel scaffold containing human serum albumin (HSA)/tri calcium phosphate nanoparticles...

full text

The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche

Azoospermia is one of the challenging disorders affecting couples who are afflicted with infertility. Human testisderivedcells (hTCs) are suitable candidates for the initiation of in-vitro spermatogenesis for these types of patients.The current study aimed to assess the proliferation of hTCs through the cell culture on the three-dimensional (3D) porousscaffolds. Cells harveste...

full text

the effect of oxytetracycline on serum calcium, phosphorus and magnesium in cattle

نتایج این مطالعه نشان می دهد که مقدار کلسیم (یونیزه و تام)، منیزیم و فسفر به طور معنی داری تغییر پیدا کرد به جزء مقدار کلسیم تام و منیزیم در روش داخل عضلانی که تغییر معنی داری نداشته است. بر اساس نتایج این مطالعه می توان نتیجه گیری کرد که اکسی تتراساکلین بر روی مقادیر سرمی کلسیم و منیزیم تأثیر می گذارد باید مقدار این کاتیون ها در بیماران، در هنگامی که اکسی تتراسایکلین استفاده می شود مورد توجه ق...

15 صفحه اول

The Effects of Plla Nanofiber Scaffold on Proliferation of Frozen-Thawed Neonate Mouse Spermatogonial Stem Cells

Purpose: To investigate of the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on proliferation of frozen-thawed neonate mouse spermatogonial stem cells.Materials and Methods: Spermatogonial cells were isolated from neonatal 3-6-day-old NMRI mice testes by two steps enzymatic digestion and differential plating. The isolated spermatogonial cells were divided into four culture groups: 1...

full text

wuthering heights and the concept of marality/a sociological study of the novel

to discuss my point, i have collected quite a number of articles, anthologies, and books about "wuthering heights" applying various ideas and theories to this fantastic story. hence, i have come to believe that gadamer and jauss are rightful when they claim that "the individaul human mind is the center and origin of all meaning," 3 that reading literature is a reader-oriented activity, that it ...

15 صفحه اول

The Homing of Spermatogonial Cells in the Cavities of a Novel Nanoscaffold

Background and Aims: In this lab trial, the effect of scaffold based on human serum albumin (HSA) and hydroxyapatite nanoparticles (HA NPs) on mouse spermatogonial cell line (SCL) was investigated. Materials and Methods: To synthesize HA NPs, calcium nitrate and diammonium phosphate at pH 13 were gently added and heated at 100 ºC for 24 hours. Then serial concentrations of HA NPs was sepa...

full text

My Resources

Save resource for easier access later


Journal title:
international journal of reproductive biomedicine

جلد ۱۳، شماره ۳، صفحات ۱۴۱-۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023